LOCUS       pEn_St_Chimera          3139 bp ds-DNA     linear       19-NOV-2015
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(2151..3011)
                     /vntifkey="4"
                     /label=AmpR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    890..924
                     /label=sgRNA
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1061..1067
                     /label=Bsu36I
                     /ApEinfo_fwdcolor=#e1e1e1
                     /ApEinfo_revcolor=#e1e1e1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    470..475
                     /label=MluI
                     /ApEinfo_fwdcolor=#e1e1e1
                     /ApEinfo_revcolor=#e1e1e1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     complement(873..878)
                     /label=BbsI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    889..892
                     /label=BbsI sticky
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    893..1020
                     /label=sgRNA(1)
                     /ApEinfo_label=sgRNA
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(1336..2003)
                     /vntifkey="33"
                     /label=Col\E1\origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     881..886
                     /label=BbsI(1)
                     /ApEinfo_label=BbsI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    476..482
                     /label=Bsu36I(1)
                     /ApEinfo_label=Bsu36I
                     /ApEinfo_fwdcolor=#e1e1e1
                     /ApEinfo_revcolor=#e1e1e1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    370..469
                     /label=attL1
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1068..1167
                     /label=attL2
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1055..1060
                     /label=MluI(1)
                     /ApEinfo_label=MluI
                     /ApEinfo_fwdcolor=#e1e1e1
                     /ApEinfo_revcolor=#e1e1e1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    622..866
                     /label=U6-26 (P) Li et al.
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    867..870
                     /label=U6-26 (P) Li et al.(1)
                     /ApEinfo_label=U6-26 (P) Li et al.
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    483..621
                     /label=U6-26 full length genomics parts
                     /ApEinfo_fwdcolor=#dbffd2
                     /ApEinfo_revcolor=#dbffd2
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    867..870
                     /label=BbsI sticky(1)
                     /ApEinfo_label=BbsI sticky
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1021..1054
                     /label=sgRNA(2)
                     /ApEinfo_label=sgRNA
                     /ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC ATTCAGGCTG CGCAACTGTT
      181 GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
      241 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
      301 ACGGCCAGTG AGCGCGACGT AATACGACTC ACTATAGGGC GAATTGGCGG AAGGCCGTCA
      361 AGGCCGCATc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      421 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctA CGCGTCCTGA
      481 GGCTTTTTTT CTTCTTCTTC GTTCATACAG TTTTTTTTTG TTTATCAGCT TACATTTTCT
      541 TGAACCGTAG CTTTCGTTTT CTTCTTTTTA ACTTTCCATT CGGAGTTTTT GTATCTTGTT
      601 TCATAGTTTG TCCCAGGATT AGAATGATTA GGCATCGAAC CTTCAAGAAT TTGATTGAAT
      661 AAAACATCTT CATTCTTAAG ATATGAAGAT AATCTTCAAA AGGCCCCTGG GAATCTGAAA
      721 GAAGAGAAGC AGGCCCATTT ATATGGGAAA GAACAATAGT ATTTCTTATA TAGGCCCATT
      781 TAAGTTGAAA ACAATCTTCA AAAGTCCCAC ATCGCTTAGA TAAGAAAACG AAGCTGAGTT
      841 TATATACAGC TAGAGTCGAA GTAGTGATTG ggGTCTTCGA GAAGACCTGT TTTTGTACTC
      901 TCAAGATTTA AGTAACTGTA CAACGAAACT TACACAGTTA CTTAAATCTT GCAGAAGCTA
      961 CAAAGATAAG gcttcatgcc gaaatcaaca ccctgtcatt ttatggcagg gtgttttttt
     1021 ctagacccag ctttcttgta caaagttggc attaACGCGT CCTGAGGacc cagctttctt
     1081 gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg aacaggtcac
     1141 tatcagtcaa aataaaatca ttatttgCTG GGCCTCATGG GCCTTCCGCT CACTGCCCGC
     1201 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAACAT GGTCATAGCT GTTTCCTTGC
     1261 GTATTGGGCG CTCTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGGTAAA
     1321 GCCTGGGGTG CCTAATGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     1381 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     1441 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     1501 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     1561 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     1621 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     1681 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     1741 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     1801 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     1861 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     1921 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     1981 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     2041 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2101 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2161 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2221 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2281 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2341 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2401 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2461 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2521 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2581 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2641 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2701 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2761 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2821 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     2881 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2941 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     3001 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3061 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     3121 TTTCCCCGAA AAGTGCCAC    
//
