All publications (chronological)

Original papers

2021 -

53. Figueira-Galán D, Heupel S, Duelli G, Tomasi Morgano M, Stapf D, Requena N  (2023)  Exploring the synergistic effects of biochar and arbuscular mycorrhizal fungi on phosphorus acquisition in tomato plants by using gene expression analyses. Sci. Total Environ., (in press)

52. Pradhan M and Requena N (2022)  Distinguishing friends from foes: Can smRNAs modulate plant interactions with beneficial and pathogenic organisms? Current Opinion in Plant Biology,

51. Fischer R and Requena N (2022)  Small secreted proteins as virulence factors in nematode-trapping fungi. Trends in Microbiology,

50. Tamayo E, Figueira-Galán D, Manck-Götzenberger J and Requena N (2022)  Overexpression of the potato monosaccharide transporter StSWEET7a promotes root colonization by symbiotic and pathogenic fungi by increasing root sink strength. Frontiers in Plant Science (in press)

49. Seemann C, Heck C, Voß S, Schmoll J, Enderle E, Schwarz D and Requena N (2022)  Root cortex development is fine-tuned by the interplay of MIGs, SCL3 and DELLA during arbuscular mycorrhizal symbiosis. New Phytologist (DOI: 10.1111/nph.17823)  

2016 -2020

48. Hartmann M, Voss S and Requena (2020) Host-Induced Gene Silencing of arbuscular mycorrhizal fungal genes via Agrobacterium rhizogenes-mediated root transformation in Medicago truncatulaMethods Mol. Biol. 2146:239-248. (doi: 10.1007/978-1-0716-0603-2_18)

47. Hause B and Requena (2020) Detection of arbuscular mycorrhizal fungal gene expression by In situ Hybridization. Methods Mol. Biol. 2146:185-196. (doi: 10.1007/978-1-0716-0603-2_14)

46. Martin FM, Dickie I, Lindahl BD, Lennon S, Öpik M, Polle A, Requena N, Selosse MA, Koide RT, Jakobsen I, Watts-Williams SJ and Cavagnaro TR (2020)  A tribute to Sally Smith. New Phytologist 228: 397-402 ( ) Virtual issue with Sally's Smith Papers (

45. Gonçalves AP, Heller J, Span EA, Rosenfield G, Do HP, Palma-Guerrero J, Requena N, Marletta M, and N. Glass L (2019)  Allorecognition upon cell-cell contact determines social cooperation and impacts the acquisition of multicellularity. Current Biology 29: 1-12 (

44. Martin FM, Harrison MJ, Lennon S, Lindahl B, Öpik M, Polle A, Requena N, Selosse MA  (2018) Cross-scale integration of mycorrhizal function. New Phytologist 220: 941-946 (

43. Voss S, Betz R, Heidt S, Corradi N and Requena N (2018) RiCRN1, a crinkler effector from the arbuscular mycorrhizal fungus Rhizophagus irregularis, functions in arbuscule development. Frontiers in Microbiology (

42. Betz R, Walter S and Requena N (2016) Alternative splicing - an elegant way to diversify the function of repeat containing effector proteins? Commentary in New Phytologist 212:306-9. (doi: 10.1111/nph.14157

41. Heck C, Kuhn H, Heidt S, Walter S, Rieger N and Requena N (2016) Symbiotic fungi control plant root cortex development through the novel GRAS transcription factor MIG1. Current Biology 26:2770-2778. (doi: 10.1016/j.cub.2016.07.059)

40. Manck-Götzenberger J and Requena N (2016) Arbuscular mycorrhiza symbiosis induces a major transcriptional reprogramming of potato SWEET sugar transporters. Frontiers in Plant Science (


2011 -2015

39. Requena N and Fischer R (2014) Breaking down walls to live in harmony. eLife Insight on Moebius et al. eLife3:e03007.

38. Doehlemann G, Requena N, Schaefer P, Brunner F, O'Connell R and Parker J (2014) Reprogramming of plant cells by filamentous plant-colonizing microbes. New Phytol., doi: 10.1111/nph.12938

37. Tisserant et al. (2013) Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosisPNAS USAdoi:10.1073/pnas.1313452110

36. Rech S, Heidt S and Requena N. (2013) A tandem Kunitz protease inhibitor (KPI106)-serine carboxypeptidase (SCP1) controls mycorrhiza establishment and arbuscule development in Medicago truncatula. Plant J. 75:711-725 

35. Tisserant et al.,  (2012) The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont. New Phytol. 193: 755-769

34. Helber N., Wippel K., Sauer N., Schaarschmidt, S., Hause B., , and Requena N. (2011) A versatile monosaccharide transporter that operates in the arbuscular mycorrhizal fungus Glomus sp. is crucial for the symbiotic relationship with plants. Plant Cell 23: 3812-3823.

33. Kloppholz S., Kuhn H. and Requena N. (2011) A secreted fungal effector of Glomus intraradices promotes symbiotic biothroph. Curr. Biol. 21: 1204-1209 (doi:10.1016/j.cub.2011.06.044)

32. Bonfante P. and Requena N. (2011) Dating in the dark: how roots respond to fungal signals to establish arbuscular mycorrhizal symbiosis. Curr. Opin. Plant Biol. (doi:10.1016/j.pbi.2011.03.014)

31. Guether M, Volpe V., Ballestrini R., Requena N., Wipf D. and Bonfante P. (2011) LjLHT1.2—a mycorrhiza-inducible plant amino acid transporter from Lotus japonicus. Biol. Fertil. Soils (in press)

2005 - 2010

30. Kuhn H., Küster H., Requena N. (2010) Membrane Steroid Binding Protein 1 induced by a diffusible fungal signal is critical for mycorrhization in Medicago truncatula. New Phytol. 185: 593-5.

29. Heupel S., Roser B., Kuhn H., Lebrun M-H., Villalba F., Requena N. (2010) Erl1 a novel ERA-like GTPase from Magnaporthe oryzae is required for full root virulence and is conserved in the mutualistic symbiont Glomus intraradices. Mol Plant Microbe Interact. 23: 67-81.

28. Martin F., Gianinazzi-Pearson V., Hijri M., Lammers P., Requena N., Sanders I.R., Shachar-Hill Y., Shapiro H., Tuskan G.A., Young J.P. (2008) The long hard road to a completed Glomus intraradices genome. New Phytol. 180: 747-750.

27. Helber, N. and Requena N. (2008) Expression of the flourescence markers DsRed and GFP fused to a nuclear localization signal in the arbuscular mycorrhizal fungas Glomus intraradices, New Phytologist. 177: 537-548.

26. Ocón A., Hampp R. and Requena N. (2007) Trehalose turnover during abiotic stress in arbuscular mycorrhizal fungi. New Phytologist 174: 879-891.

25. Kuster H., Becker A., Firnhaber C., Hohnjec N., Manthey K., Perlick A.M., Bekel T., Dondrup M., Henckel K., Goesmann A., Meyer F., Wipf D., Requena N., Hildebrandt U., Hampp R., Nehls U., Krajinski F., Franken P., Puhler A. (2007) Development of bioinformatic tools to support EST-sequencing, in silico- and microarray-based transcriptome profiling in mycorrhizal symbioses. Phytochemistry 38: 19-32.

24. Requena N., Serrano E., Ocon A., Breuninger M. (2007) Plant signals and fungal perception during arbuscular mycorrhiza establishment. Phytochemistry, 68:33-40.

23. Cruz C., Egsgaard H., Trujillo C., Ambus P., Requena N., Martins-Loução M.A. and Jakobsen I. (2007) Enzymatic Evidence for the key role of arginine in nitrogen translocation by arbuscular mycorrhiza fungi, Plant Physiol. 144: 782-792.

22. Requena N. (2005) Measuring quality of service: Phosphate "a la carte" by arbuscular mycorrhizal fungi. New Phytologist 168: 268-271.

21. Requena N. (2005) Molekulare Analyse der fruehen Stadien der Arbuskulaeren Mycorrhizasymbiose. Biospektrum Sonderaufgabe, 11 Jahrgang, pp. 539.

2001 - 2004

20. Breuninger M. and Requena N. (2004) Recognition events in AM symbiosis: Analysis of fungal gene expression at the early appressorium stage. Fungal Genet. Biol. 41: 794-804.

19. Breuninger M., Trujillo C.G., Serrano E., Ecke M., Fischer R. and Requena N. (2004) Different N sources modulate activity but not expression of glutamine synthetase in two arbuscular mycorrhizal fungi. Fungal Gen. Biol. 41: 542-552.

18. Wei H., Vienken K., Weber R., Bunting S., Requena N. and Fischer R. (2004) A putative high affinity transporter, hxtA, of Aspergillus nidulans is induced in vegetative hyphae upon starvation and in ascogenous hyphae during cleistothecium formation. Fungal Genet. Biol. 41:148-156.

17. Requena N., Breuninger M., Franken P. and Ocón-Garrido A. (2003) Symbiotic status, phosphate and sucrose regulate the expression of two plasma membrane H+-ATPase genes from the mycorrhizal fungus Glomus mosseae Plant Physiol 132: 1540-1549.

16. Wei H., Requena N. and Fischer R. (2003) The MAPKK-kinase SteC regulates conidiophore morphology and is essential for heterokaryon formation and sexual development in the homothallic fungus Aspergillus nidulans. Mol. Microbiol. 47: 1577-1588.

15. Requena N., Mann P., Hampp and Franken P. (2002) Early developmentally regulated genes in the arbuscular mycorrhizal fungus Glomus mosseae: GmGIN1, a novel gene encoding a protein with homology to the C-terminus of metazoan hedgehog proteins. Plant Soil 244: 129-139.

14. Requena N., Alberti-Segui C., Winzenburg E., Horn C., Schliwa M., Philippsen P., Liese R. and Fischer R. (2001) Genetic evidence for a microtubule-destabilizing effect of conventional kinesin and analysis of its consequences for the control of nuclear distribution in Aspergillus nidulans. Mol. Microbiol. 42:121-132.

13. Franken P. and Requena N. (2001) Analysis of gene expression in arbuscular mycorrhizas: new approaches and challenges. Research Review, New Phytol 150: 517-523.

12. Requena N., Pérez-Solis E., Azcón-Aguilar C, Jeffries P. and Barea J. M. (2001) Increased establishment of mycorrhizal plants inoculated with native endophytes in semi-arid degraded mediterranean ecosystems. Appl. Environ. Microbiol. 67: 495-498. (selected in the „Journal Highlights“ of the American Society of Microbiology News Journal, ASM News 67 (4), 2001)

1999 - 2000

11. Requena N., Mann P. and Franken P. (2000) A homologue of the cell-cycle check-point TOR2 from yeast exists in the arbuscular mycorrhizal fungus Glomus mosseae. Protoplasma 211: 89-98.

10. Franken P., Requena N., Bütehorn B., Krajinski F., Kuhn G., Lapopin L., Mann P., Rhody D. and Stommel M. (2000) Molecular analysis of the arbuscular mycorrhiza symbiosis. Arch. Agron. Soil Sci. 45: 271-286.

9. Herrera J.A., Caballero-Mellado J., Laguerre G., Tichy H.-V., Requena N., Amarger N., Martínez-Romero E., Olivares J., and Sanjuan J. (1999) At least five rhizobial species nodulating Phaseolus vulgaris in a Spanish soil. FEMS Microbiol. Ecol. 30: 87-97.

8. Requena N., Füller P. & Franken P. (1999) Molecular characterization of GmFOX2 an evolutionarily highly conserved gene from the mycorrhizal fungus Glomus mosseae, down-regulated during interaction with rhizobacteria. Mol. Microb. Plant Int. 12: 934-942.

1996 - 1997

7. Requena N. (1997) Mycorrhizal symbiosis and management of the mycorrhizosphere as a tool for the restoration of degraded semi-arid ecosystems. Recent Res. Develop. Microbiol. 1: 267-276.

6. Tisserant B., Brenac V., Requena N., Jeffries P. & Dodd J.C. (1997) The detection of Glomus spp. (arbuscular mycorrhizal fungi) forming mycorrhizas in three plants, at different stages of seedling development using mycorrhiza-specific isozymes. New Phytol. 138: 225-239.

5. Requena N., Jiménez I., Toro M. & Barea J.M. (1997) Rhizospheric interactions between PGPR, AMF and Rhizobium from semi-arid ecosystems on Anthyllis cytisoides l. A model legume for revegetation studies. New Phytol. 136: 667-676.

4. Requena N., Baca M.T. & Azcón R. (1997) Evolution of humic substances from compost during incubation wiht ligno-cellulolytic microorganisms and effects in lettuce plants inoculated with Azotobacter chroococcum. Biol. Fertil. Soils 24: 59-65.

3. Requena N., Azcón R. & Baca M.T. (1996) Chemical changes in humic substances from compost due to incubation with ligno-cellulolytic microorganisms and effects on lettuce growth. Appl. Microbiol. Biotechnol. 45: 857-863.

2. Barea J.M., Requena N. & Jiménez I. (1996) Desertification control: a strategy based on the managment of microorganisms to recover desertified mediterranean ecosystems. Options Mediterraneennees. Serie Cahiers. CIHEAM 20: 75-86.

1. Requena N., Jeffries P. & Barea J.M. (1996) Assessment of natural mycorrhizal potential in a desertified semi-arid mediterranean ecosystem. Appl. Environ. Microbiol. 62: 842-847.