GATEWAY Vectors
General
GATEWAY is a modular system of vectors for plant transformation. The construct of interest is first introduced into the entry vector, and can from there be shuffled into different destination vectors (different FP versions, different selection markers, different placement of the FP on the protein) in a simple recombination reaction.
The vectors are administrated by Nadja Wunsch, aliquots can be obtained by her, midipreps have to be done by the users themselves.
The list is ordered by purpose
- 1. donor vector
- 2. FP fusions for stable transformation
- 3. FP fusions for transient transformation
- 4. other applications
1. Donor vector (Entry vector)
| Reference number | Designation | Information |
| GW-V1 | pDONRZeo | entry for the system, map detailed information |
2. FP fusions for stable transformation
| Colour | Position | Resistance | Designation | Accession Number | Remarks |
| GFP | N-term | KanR | pK7WGF2,0 | GW-V13 | map |
| GFP | C-term | KanR | pK7FWG2,0 | GW-V12 | map |
| GFP | N-term | HygR | pH7WGF2,0 | GW-V18 | map |
| GFP | C-term | HygR | pH7FWG2,0 | still lacking | map |
| GFP | N-term | BastaR | pB7WGF2,0 | GW-V34 | map |
| GFP | C-term | BastaR | pB7FWG2,0 | still lacking | map |
| RFP | N-term | KanR | pK7WGR2,0 | still lacking | map |
| RFP | C-term | KanR | pK7RWG2,0 | still lacking | map |
| RFP | N-term | HygR | pH7WGR2,0 | GW-V11 | map |
| RFP | C-term | HygR | pH7RWG2,0 | GW-V10 | map |
| RFP | N-term | BastaR | pB7WGR2,0 | GW-V35 | map |
| RFP | C-term | BastaR | pB7RWG2,0 | still lacking | map |
| GW-V14 | pK7WGY2,0 (YFP, kan) | midiprep 1,5 µg/µl (4.4.06) | |||
| GW-V15 | pK7WGC2,0 (CFP, kan) | midiprep 1,7 µg/µl (4.4.06) | |||
| GW-V16 | pK7YWG2,0 (YFP, kan) | midiprep | |||
| GW-V17 | pHGWFS7 (promoter analysis, hyg) | midiprep 1,4 µg/µl (23.10.06) | |||
| GW-V19 | pH7WGY2,0 (YFP, hyg) | midiprep 0,34 µg/µl (25.5.07) | |||
High-copy vectors for transient transformation
| Reference number | Designation | Information | Fluorescence |
| GW-V20 | p2GWR7 (RFP) | midiprep 1,19 µg/µl (7.12.07) - map |
|
| GW-V21 | p2RGW7 (RFP) | midiprep 1,15 µg/µl (7.12.07) - map |
|
| GW-V22 | p2GWF7 (GFP) | midiprep 0,35 µg/µl (27.10.05) - map |
|
| GW-V23 | p2FGW7 (GFP) | midiprep 2,80 µg/µl (21.7.05) - map |
|
| GW-V24 | p2GWY7 (YFP) | midiprep 0,77 µg/µl (27.10.05) - map |
|
| GW-V25 | p2YGW7 (YFP) | midiprep 0,45 µg/µl (27.10.05) - map |
|
| GW-V26 | p2GWC7 (CFP) | midiprep 0,30 µg/µl (27.10.05) - map |
|
| GW-V27 | p2CGW7 (CFP) | midiprep 0,39 µg/µl (27.10.05) - map |
|
| GW-V30 | pMAV GW YC | midiprep 1,93 µg/µl (10.8.05) |
|
| GW-V31 | pMAV GW YN | midiprep 0,58 µg/µl (10.8.05) |
|
| GW-V32 | pMAV YC GW | midiprep 1,76 µg/µl (15.8.05) |
|
| GW-V33 | pMAV YN GW | midiprep 1,85 µg/µl (10.8.05) |
|
Entry vectors
| Reference number | Designation | Information | Insert |
| GW-E01 | pENTR Arp3 ÅSTOP | 0,22 µg/µl (28.08.05) | tobacco actin-related protein 3 (Jan Maisch) |
| GW-E02 | pENTR FABD2 ÅSTOP | 0,15 µg/µl (22.07.05) | actin-binding domain Ath-fimbrin (Jan Maisch) |
| GW-E03 | pENTR KGAP1 (w/o stop) | 0,58 µg/µl (08.11.05) | interactor of Ath RanGAP1 (Petra Hohenberger) |
| GW-E04 | pENTR KGAP1ÅSTOP | interactor of Ath RanGAP1 (Petra Hohenberger) | |
| GW-E05 | pENTR Mya2ÅSTOP | tail of class XI myosin AthMya2 (Carola Holweg) | |
| GW-E06 | pENTR Mya2-DILÅSTOP | 0,92 µg/µl (08.11.05) | tail class XI myosin AthMya2, truncated DIL (Carola Holweg) |
| GW-E07 | pENTR RanGAP1ÅSTOP | Ath RanGAP1 (Petra Hohenberger) | |
| GW-E08 | pENTR Tua3ÅSTOP | 174 ng/µl (3.5.07) | tobacco tubulin a3 (Petra Hohenberger) |
| GW-E09 | pENTR RanGAP1 (w/o stop) | Ath RanGAP1 (Petra Hohenberger) |
Expression vectors
| Reference number | Designation | Information | Insert | Fluorescence | Resistance |
| GW-X01 | pK7WGC2 FABD2 | 1,1 µg/µl (4.4.06) | Fimbrin, stabil | Kan | |
| GW-X02 | pK7WGY2 FABD2 | 0,96 µg/µl (4.4.06) | Fimbrin, stabil | Kan | |
| GW-X03 |
pK7WGF2 FABD2 |
2 µg/µl (26.07.05) | Fimbrin, stabil | Kan | |
| GW-X04 | p2FGW7 FABD2 | 2 µg/µl (26.07.05) | Fimbrin | transient | |
| GW-X05 |
p2RGW7 FABD2 |
0,8 µg/µl (06.12.05) | Fimbrin | transient | |
| GW-X06 | pK7WGF2 Arp3 | 1,8 µg/µl (02.09.05) | NtArp3 | Kan | |
| GW-X07 | p2FGW7 Arp3 | 1,7 µg/µl (02.09.05) | NtArp3 | transient | |
| GW-X08 |
p2RGW7 Arp3 |
0,68 µg/µl (06.12.05) | NtArp3 | transient | |
| GW-X09 |
pH7WGF TuA3 ÅSTOP |
1,1 µg/µl (13.6.07) | NtTuA3 | Hyg |