Constructs: Kinesins
The entire list of constructs can be found here. For many constructs there are data sheets with details. Aliquots of the constructs can be obtained from Sabine Purper giving the code
|
Accession |
what is it |
use |
data sheet |
| Rice KCH1 | survey of tools for OsKCH1 | ||
|
K001 |
OsKCH1fl-Entry | Entry vector full-length for GATEWAY cloning | |
| K002 | OsKCH1mot-Entry | Entry vector motor domain for GATEWAY cloning | |
| K003 | OsKCH1ch-Entry | Entry vector CH domain for GATEWAY cloning | |
| K004 | OsKCH1fl-His | full length, recombinant expression | |
| K005 | OsKCH1mot-His | motor domain, recombinant expression | |
| K006 | OsKCH1ch-His | CH domain, recombinant expression | |
| Rice DLK | |||
| GATEWAY entry constructs | |||
| K021 | pDONR-DLK (C) | Entry vector full-length for GATEWAY cloning without stop codon (1-2292 bp) | |
| K022 | pDONR-DLK (N) | Entry vector full-length for GATEWAY cloning with stop codon (1-2295 bp) | |
| K023 | pDONR-DLK-motor (C) | Entry vector motor domain for GATEWAY cloning without stop codon (1111-2292 bp) | |
| K024 | pDONR-DLK-motor (N) | Entry vector motor domain for GATEWAY cloning with stop codon (1111-2295 bp) | |
| GFP-fusions | |||
| K025 | pK7FWG2.0-DLK | DLK full length (1-2292 bp) with GFP fused to its C-terminus, KanR | |
| K026 | pK7WGF2.0-DLK | DLK full length (1-2295 bp) with GFP fused to its N-terminus, KanR | |
| K027 | pK7FWG2.0-DLK-motor | DLK motor domain (1111-2292 bp) with GFP fused to its C-terminus, KanR | |
| K028 | pK7WGF2.0-DLK-motor | DLK motor domain (1111-2295 bp) with GFP fused to its N-terminus, HygR | |
| RFP-fusions | |||
| K025 | pH7RWG2.0-DLK | DLK full length (1-2292 bp) with RFP fused to its C-terminus, HygR | |
| K026 | pH7WGR2.0-DLK | DLK full length (1-2295 bp) with RFP fused to its N-terminus, KanR | |
| K027 | pH7RWG2.0-DLK-motor | DLK motor domain (1111-2292 bp) with RFP fused to its C-terminus, KanR | |
| K028 | pH7WGR2.0-DLK-motor | DLK motor domain (1111-2295 bp) with RFP fused to its N-terminus, HygR | |
| recombinant expression | |||
| K029 | pDEST42-DLK-Tail | DLK tail (1-1209bp) with His tag fused to its C-terminus |