Glossar Fluoreszenzmikroskopie und Fluoreszente Sonden
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|Artefakt (arte factum)
|False experimental data produced by problems of methodology or equipment
|Bimodal Fluorescence Complementation (BiFC)
Fluorescence produced by two interaction partners fused to the two halves of a split YFP
from Bio-Ballistics, shooting foreign DNA into cells (can be transient or stable, depending on the vector)
|Conversion of chemical energy into light (does not require excitation!), e.g. Aequorin
|Boron-DiPyrrromethene based fluorochromes with superior optical properties
|codon usage bias
|Asymmetric occurrence of the different tRNAs conjugated to a given amino acid residue, organism-specific
|dichroitic mirror, beam splitter
|dichroitischer Spiegel, Farbteiler
|Filter that reflects or transmits light depending on wavelength
|Change in the direction of a light beam upon transition between different optical density (diffraction index)
|The smallest distance that can be resolved by light microscopy. According to Abbé's Law this limit is defined as ratio of wavelength by numerical aperture.
|Minimal auxin-responsive promotor made of a 5' mutation of auxin-response element D1-4 of the gene GH3
|Generation of light by an excited fluorochrome by transition from the singulet back to the ground state
|Microscopical set-up, where the excitation light is administered through the objective to the specimen
|Generation of a weak wave-front during total reflection at the border between different diffraction indices
|Transition of a fluorescent molecule from the ground state into the singulet-state by absorption of a photon
|Molecule that is excited for fluorescence in the visible range
|Fluorescent Recovery After Photobleaching - approach to measure protein dynamics by bleaching a ROI
|stands for fluorescence resonance energy transfer (irradition-free energy transfer between a pair of fluorochromes)
|product of sin a (opening angle of objective) and diffraction index n (1 for air, 1.41 for glass)
|manipulation of small structures by a strong laser beam, based on gradients in diffractive index
|Fluorochrome, where fluorescence properties can be switched by light different from fluorescence
|Small hole in confocal laser microscopy filtering light depending on its geometrical relation with the focal plane
Specific molecules that are used to investigate a specific biological process
|Approach in quantitative image analysis, where pixel intensities obtained at two emission wavelengths are divided to remove the influence of intensity changes not caused by the fluorochrome of interest
|Gene that is easily detected and reports the activitiy of the promotor under whose control it was placed
|Region of interest which is in the focus of investigation
|Scanning Electron Microscopy, the surface of a sample is scanning using the signal of backscattered electrons
|stands for stimulated emission depletion, a specimen is excited by a laser and the excitation is quenched by a second laser. Due to interference of the laser waves, it is possible to generate an excitation spot that is smaller than the diffraction limit
|approach to use interference patterns for excitation that can be smaller than the diffraction limit.
|In fluorescence shift of the emitted light to lower energy (longer wavelength), caused by dissipation of heat during transition between different singulet states
|stands for 3D stochastic optical reconstruction microscopy, approach to reconstruct the exact position beyond the diffraction limt by using temporal information of a photoactivable fluorochrome.
|stands for total-internal reflection microscopy using the fact that an evanescent field can penetrate only a very short distance into the sample such that only the uppermost 50-100 nm are excited.
|two coupled lasers of low energy are focussed such that energy is added up in the focus site
|visualise / visualize
|to render a protein or structure visible, usually by specific labels