What we expect you to know

Terminology

You should know, what these terms mean and you should be able to explain them in your own words.

Compartments . ER . Golgi . Exocytosis . Endocytosis . Trans Golgi Network . Multivesicular Body . Exosomes . Clathrin . Cotranslational and posttranslational transport . vSNARES . tSNARES . Cellulose synthase . Microfibril

German-English Glossary

 

 

 

Content

 

  • You can explain, what compartments are, and what they are good for

  • You can sketch down the path of an organelle and of a secreted protein

  • You can name one animal and one plant example for signalling through vesicle transport

  • You can explain, how the cell wall is synthetised and what are old and young layers

  • You can explain, how polar auxin transport is generated

 

Vertiefung (for Bachelor students)

  • 1. Is a nucleus a compartment? Justify your answer
  • 2. A small ribosomal subunit from spinach needs 10 hours in your centrifugation set-up to reach its final destination. How long does the large ribosomal subunit need?
  • 3. In a study on a novel peptoid targeting mitochondria, we observed that Wortmannin blocked the uptake completely, while Ikarugamycin did not block uptake. What is your explanation?
  • 4. The fungus Botrytis cinerea secretes exosomes with miRNA to block plant defence. As charged molecule, miRNA cannot pass the plant membrane. How does this work then?
  • 5. How would the shape of a root hair change, when you destroy actin filaments with the inhibitor Latrunculin B?
  • 6. Plasmolysis is often used as test to find out, whether a plant cell is alive? Why does this work?

 

Special topic (for Master students): see Ilias course

 

Read more

  • Cui Y, Gao JY, He YL, Jiang LW (2020) Plant extracellular vesicles. Protoplasma 257, 3-12. more... (can be downloaded when you are in the KIT net or connected by vpn).